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(a) Representative images of stained skin with tissue with hematoxylin and eosin or Masson’s trichrome (MT). Arrows indicate microfolds in skin epithelial surface. Scale bars indicate 200 µm. (b) Immuno-stained skin tissue using nitrotyrosine (NT), 4-hydroxynonenal (4-HNE), cleaved caspase-3, cleaved PARP, and MMP9 antibodies. Scale bars indicate 100 µm. EP, epithelium; DE, dermis; CM, cutaneous muscle; SE, sebaceous gland; AC, adipocyte; Th, thickness.
