Fig. 3.

Live S. gordonii activates inflammasomes in IFNγ-stimulated macrophages. (A, B) Percent ASC speckle–positive cells after incubation (A: 6 h; B: 12 h) with live or heat-killed (HK) S. gordonii in PMA-differentiated THP-1 Asc-GFP cells polarized with IFNγ. Positive control: 4 h 1 µg/mL LPS + 30 min 20 µM nigericin. (C) Representative image of ASC speckle–positive cells. (D) Representative image of ASC speckle–positive cell containing S. gordonii. Shown are mean ± SEM of at least 4 independent experiments in which at least 1,000 cells per condition were analyzed. P values were calculated by ordinary 1-way analysis of variance followed by Tukey's multiple comparisons test. Sg = Streptococcus gordonii.