Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 20;621(7980):857–867. doi: 10.1038/s41586-023-06549-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 10 — a, Ingenuity Pathway Analysis (IPA) of differential gene expression data from ionocyte subtypes. The charts represent the top significantly associated canonical pathways. P value was calculated using a right-tailed fisher’s exact test. b, (Left) RNAscope validation of Type A, Type B or Type C ionocytes from a cytospun ferret ALI culture. Representative images of n = 10 ionocytes. (Right) Immunofluorescence validation of Type A, Type B or Type C ionocytes in a section of human ALI culture. BSND positive ionocytes are Type A subtype, some of which co-express FOXI1 (yellow arrows) or lack FOXI1 expression (red arrows). BSND negative ionocytes that express FOXI1 (green arrows) are Type B or Type C subtype. Intermediated expression of FOXI1 in BSND positive cells is marked by overlapping yellow and red arrows. Right panels show nuclei in white to demarcate the cell more clearly. Representative images of n = 7 ALI cultures. c,d, Lineage tracing of FOXI1-Cre^ERT2::ROSA-TG ALI culture following hydroxytamoxifen (TAM) treatment on (c) day 1–17 or (d) day 14–22 produce different proportions of EGFP⁺ATP6V1G3⁺ ionocytes and EGFP⁺ATP6V1G3^– other rare cell types. Yellow and green arrowheads indicate traced ionocytes or traced non-ionocytes, respectively. Representative image from n = 16 independent samples from TAM day 1–17 group and n = 15 independent samples from TAM day 14–22 group. e, Percentage of FOXI1-Cre^ERT2 traced EGFP⁺ cells that also expressed ATP6V1G3 from scRNA-seq and the two TAM treatment protocols (Mean ± s.e.m.; n = 8 independent samples in scRNA-seq group, n = 16 independent samples from 4 different animals in ALI TAM Day 1–17 group, n = 15 independent samples from 4 different animals in ALI TAM Day 14–22 group; P value determined by one-way ANOVA and Tukey’s multiple comparisons test). f,g, Lineage tracing of PNECs in actively differentiating FOXI1-Cre^ERT2::ROSA-TG basal cells treated with TAM on day 1–17 of ALI culture. Cultures were immunostained with PNEC marker SYP on day 21. Yellow, red, and green arrowheads indicate traced PNEC, untraced PNEC, or traced non-PNEC, respectively. Representative image of 11 independent cultures. h, Cell-cell Pearson correlation coefficient matrix (r, color bar) across species (human, mouse, ferret) ordered by cluster assignment (tuft, PNEC, ionocyte).

Source Data