. 2023 Sep 20;621(7980):857–867. doi: 10.1038/s41586-023-06549-9

Table 1.

Genomic information on CRISPR–Cas9 zygote targeting

Ferret line	sgRNA sequence (PAM)	sgRNA genomic cleavage site*	Length of homology arms	Genomic site of homology arms^a
FOXI1-KO	gRNA 1 5′-TGGTAAAGGCTCATCTCGGGG-3′ gRNA 2 5′-GCGGCCCCCCTATTCCTACTCGG-3′	gRNA 1: 72–95 gRNA 2: 368–391	NA	NA
FOXI1-Cre^ERT2	gRNA 5′-CTAGACCTCGGTGCCCTCCCTGG-3′	sgRNA: 2,579–2,602	Right: 952 bp Left: 967 bp	Right: 2,603–3,554 Left: 1,635–2,602
CFTR^L/L	gRNA-right: 5′-TAGCTAAATCCTTTGGGAACTGG-3′ gRNA-left: 5′-TATTTCCTGTTGAATGATGGAGG-3′	gRNA-right: 108,071–108,093 gRNA-left: 107,612–107,631	Right: 777 bp Left: 703 bp	Right: 108,077–108,853 Left: 106,922–107,624

Ferret line

sgRNA sequence (PAM)

sgRNA genomic cleavage site*

Length of homology arms

Genomic site of homology arms^a

FOXI1-KO

gRNA 1

5′-TGGTAAAGGCTCATCTCGGGG-3′

gRNA 2

5′-GCGGCCCCCCTATTCCTACTCGG-3′

gRNA 1: 72–95

gRNA 2: 368–391

FOXI1-Cre^ERT2

gRNA

5′-CTAGACCTCGGTGCCCTCCCTGG-3′

sgRNA: 2,579–2,602

Right: 952 bp

Left: 967 bp

Right: 2,603–3,554

Left: 1,635–2,602

CFTR^L/L

gRNA-right:

5′-TAGCTAAATCCTTTGGGAACTGG-3′

gRNA-left:

5′-TATTTCCTGTTGAATGATGGAGG-3′

gRNA-right:

108,071–108,093

gRNA-left:

107,612–107,631

Right: 777 bp

Left: 703 bp

Right: 108,077–108,853

Left: 106,922–107,624

^aENSEMBL contig and bp length: CFTR ENSMPUG00000007138, 169,387 bp; FOXI1 ENSMPUG00000012982, 2,602 bp.

bp, base pair; gRNA, guide RNA; NA, not applicable; PAM, protospacer adjacent motif; sgRNA, single-guide RNA.