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. 2023 Sep 13;621(7980):877–882. doi: 10.1038/s41586-023-06528-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Representative 4-12% Bis-Tris SDS-PAGE gel (of three purifications) stained with Coomassie blue, indicating elution of the GluA1/γ3 and GluA2(F231A)/γ2 (Gly743) complex from FLAG beads. For gel source data, see Supplementary Fig. 1. b, Representative motion-corrected micrograph of resting-state GluA1/γ3 (scale bar, 50 nm). c, Representative 2D class averages of resting state GluA1/γ3. d, General cryo-EM data processing workflow used for all data sets in this work. Raw movies were first motion-corrected in RELION (blue), and generated micrographs were then imported into CryoSPARC (black) for CTF estimation, particle picking, and cleaning. Selected particles were converted and imported back into RELION for Bayesian polishing, CTF refinement, and further 3D refinement. Refined or polished particles as appropriate were then returned to CryoSPARC for non-uniform refinement and 3D variability analysis. e, Focused refinement and classification scheme used for data sets in this work. Focused refinement on LBD-TMD or TMD regions was performed to improve the resolution. Classifications on TARP loops and NTD were performed by using soft masks on those regions or doing particle subtraction.