Figure 3.

NeuroMabSeq workflow with details of samples sequenced. This Sankey diagram depicts the details of hybridoma samples sequenced to date. The 8642 novel (i.e., non-control hybridoma) samples sequenced included 1903 that did not yield usable sequences and were designated as “dropouts”. 6739 samples returned usable sequences that yielded 15,064 total V_L and V_H sequences. 13,401 of these remained after eliminating any sequences that had insufficient support, did not conform to ANARCI conventions of valid antibody sequences, or were duplicates; these were subjected to the star scoring system. Of these, 11,226 had sequencing quality scores greater than 3, while 2175 samples had sequencing quality scores less than 3. The number of unique mAb IDs after grouping all biological and technical replicates is also provided (1931 high quality and 331 low quality).