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. 2023 Sep 27;13:16200. doi: 10.1038/s41598-023-43233-4

Figure 6.

Figure 6

R-mAb and scFv Gibson Assembly cloning strategy. (a) R-mAb cloning strategy showing a schematic of the four-piece Gibson Assembly based construction of the R-mAb expression plasmid and at the bottom a schematic of the VL and VH gene fragments. (b) scFv cloning strategy showing a schematic of the two-piece Gibson Assembly based construction of the scFv expression plasmid and at the bottom a schematic of the VH -linker- VL gene fragment.