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. 2023 Sep 27;13:16200. doi: 10.1038/s41598-023-43233-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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R-mAb evaluation. (a) Immunofluorescence immunocytochemistry on transiently transfected COS-1 cells. Cells were transfected with a plasmid encoding mouse THIK-2 and double immunolabeled with the progenitor N468/37 IgG1 mAb (green) and the N468/37R IgG2a R-mAb (red). Hoechst nuclear labelling is shown in blue. (b) Immunoperoxidase/DAB immunohistochemistry on rat brain sections comparing immunolabeling with the anti-HCN4 hybridoma-generated N114/10 mAb to the N114/10R R-mAb. (c) Strip immunoblots on rat brain samples showing side-by-side comparisons of hybridoma-generated conventional progenitor mAb samples “C” and transfected cell generated R-mAb samples “R”. Numbers to the left of each set denote mobility of molecular weight standards in kD. (d) Immunofluorescence immunohistochemistry on rat brain sections. Multiplex immunolabeling with four mouse mAbs including the subclass-switched R-mAb L113/130R in adult rat hippocampal CA1.