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. 2023 Sep 27;13:16200. doi: 10.1038/s41598-023-43233-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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scFv evaluation. (a) Immunofluorescence immunocytochemistry on transiently transfected COS-1 cells. Cells were transfected with a plasmid encoding the mouse GABAB R1 receptor and double immunolabeled with the progenitor N93A/49 mouse mAb (green) and the N93A/49 scFv (red). Hoechst nuclear labelling is shown in blue. (b) Immunoperoxidase/DAB immunohistochemistry on rat brain sagittal sections. The top pair of images show immunolabelling with the anti-GABA_A receptor α4 hybridoma-generated N398A/34 mAb (top section) and the N398A/34 scFv (bottom section). The bottom pair of images show immunolabelling in neocortex with the anti-Ankyrin-G hybridoma-generated N106/20 mAb (left section) and the N106/20 scFv (right section). (c) Immunofluorescence immunohistochemistry on rat brain sagittal sections. The top set of images show multiplex immunolabeling of brain (top two rows) and hippocampus (bottom row) with the anti-VGlut1 hybridoma-generated N28/9 mAb (IgG1) (left section) and the N28/9 scFv (right section) in green. The sections were also labelled with the mouse mAbs anti-Parvalbumin L114/3 mAb (IgG2a) in red and the anti-Amigo-1 L86A/37 mAb (IgG2b) in purple. Hoechst nuclear labelling is shown in blue. The bottom pair of images show multiplex immunolabeling of rat cerebellum with the anti-Kv1.2K⁺ channel hybridoma-generated K14/16 mAb (IgG2b) (left section) and the K14/16 scFv (right section) in green. The sections were also labelled with the mouse anti-Calbindin L109/39 mAb (IgG2b). Hoechst nuclear labelling is shown in blue.