Table 1.

Sequencing summary after scoring and filtering.

Category	Total count	Total percent (%)	Chemical fusion count	Chemical fusion percent (%)	Electrofusion count	Electrofusion percent (%)
VL or VH = 0	341	17.7	186	17.2	155	18.3
Total (VL ≥ 1 and VH ≥ 1)	1590	82.3	896	82.8	694	81.7
No additional chain	1553	80.4	874	80.0	679	80.0
Additional chains of some sort	37	1.9	22	2.0	15	1.8
One additional chain of some sort	37	1.9	22	2.0	15	1.8
One additional VL only	23	1.2	14	1.3	9	1.1
One additional VH only	4	0.2	2	0.2	2	0.2
One additional VL and VH	10	0.5	6	0.6	4	0.5

Total number of high quality unique mAb ID sequences (1931 total) from projects performed using PEG chemical fusion (1082 total) and electrofusion (849 total) to generate the hybridomas. Samples with at least one V_L and one V_H sequence are then separated into those containing exactly one V_L and one V_H sequence, one additional chain of some sort, one additional V_H, one additional V_L, and one additional V_L and V_H. Projects performed using either PEG chemical fusion or electrofusion were then analyzed separately.