Fig. 2. IAV PPI networks from three cell types identify strain-specific and pan-IAV-human interactions.

High-confidence IAV-human PPIs between 12 IAV proteins (grey diamonds) and 214 human proteins (circular nodes) identified from three IAV strains unified across the three cell types. Human protein nodes are split into three sections and colored by the IAV strain for which the interaction was identified: pH1N1 (blue), H3N2 (green) and H5N1 (purple). Color shading is proportional to MiST PPI confidence score (scale at bottom; not identified represented by white color), enabling visualization of high-confidence interactions that scored above our MiST score thresholds and interactions with additional IAV strain(s) detected in our AP-MS data that fell below our MiST score thresholds. For PPIs that are shared between multiple IAV proteins or cell types, the maximum MiST score from either IAV protein or cell type is reported in the network for each strain. IAV-human PPIs are depicted (dark grey lines), and human-human PPIs are identified (light grey lines) as curated in the CORUM¹³⁰ database. Human protein complexes (yellow halo) are labeled as described in CORUM¹³⁰, and biological processes (pink halo) are labeled as described by GO terms.