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. 2023 Jul 17;60(11):6441–6465. doi: 10.1007/s12035-023-03456-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Serpine-1 is released in EVs from control, Aβ, and/or HIV-1 exposed brain endothelial cells (A) Visualization by confocal microscopy of Serpine-1 GFP (green), CD63 RFP (red), and Aβ (1–40) HiLyte (yellow) (arrow heads) in EVs isolated from media of transfected and treated HBMEC as in Fig. 1. Examples of overlapping fluorescence of Serpine-1 GFP, CD63 RFP, and/or Aβ (1–40) HiLyte associated with EVs are indicated by arrows. DAPI stains the genetic material in EVs. Representative images from three experiments. Scale bar: 5 μm. B Quantification of Serpine-1 GFP-positive, CD63 RFP-positive, and Serpine-1 GFP/CD63 RFP-double positive EVs. Values are mean ± SEM, n = 5–7. One- and two-way ANOVA with Šídák's and Tukey’s multiple comparisons tests. *Statistically significant at p < 0.05

Fig. 2 — Serpine-1 is released in EVs from control, Aβ, and/or HIV-1 exposed brain endothelial cells (A) Visualization by confocal microscopy of Serpine-1 GFP (green), CD63 RFP (red), and Aβ (1–40) HiLyte (yellow) (arrow heads) in EVs isolated from media of transfected and treated HBMEC as in Fig. 1. Examples of overlapping fluorescence of Serpine-1 GFP, CD63 RFP, and/or Aβ (1–40) HiLyte associated with EVs are indicated by arrows. DAPI stains the genetic material in EVs. Representative images from three experiments. Scale bar: 5 μm. B Quantification of Serpine-1 GFP-positive, CD63 RFP-positive, and Serpine-1 GFP/CD63 RFP-double positive EVs. Values are mean ± SEM, n = 5–7. One- and two-way ANOVA with Šídák's and Tukey’s multiple comparisons tests. *Statistically significant at p < 0.05