FIGURE 6.
miR‐146a‐5p knockdown in MBM cells significantly decreased tumour burden and increased survival in a mouse brain metastasis model. (a) 5 × 105 H1_DL2 miR‐NC KD (control) or H1_DL2 miR‐146a‐5pKD cells were injected intracardially into mice. Tumour burden was evaluated at week 4 and 6 with IVIS bioluminescent imaging. n = 10 mice per group. (b) Tumour distribution in head and body were quantified at week 4 and 6 by the average radiance in photons/s/cm2/steradia (p/s/cm2/sr). (c) Kaplan–Meier survival curves calculated for all animals in the treatment study. (d) Representative images of Ki67 staining of FFPE sections of brain tumour tissue from animal test subjects after sacrificing. Scale bar = 50 μm. (e) Quantification of Ki67 staining as a percentage of total cells in each tumour. n.s.= not significant, *p < 0.05, **p < 0.01.