FIG. 1.

The IS6110-based PCR patterns of all investigations with discrepant results and some isolates with a low number of IS6110 copies are shown following agarose gel electrophoresis on a 1% (wt/vol) agarose gel. Discrepant isolates are shown in lanes as follows: lanes 1 to 8, investigation 3, isolates 3a to 3h; lanes 9 to 14, investigation 10, isolates 10a to 10f; lanes 15 and 16, investigation 15, isolates 15a and 15b; lanes 17 and 18, investigation 16, isolates 16a and 16b; and lanes 19 to 25, investigation 21, isolates 21a to 21g. Low-IS6110-copy-number isolates are shown in lanes as follows: lanes 26 and 27, investigation 9, isolates 9a to 9b (two copies of IS6110), and lane 28, investigation 7, isolate 7a (one copy of IS6110). S, DNA size standards (in base pairs) are indicated in the margin.