Table 11.
Proteins which are uniquely absent in E. faecalis when grown in the presence of gliotoxin (5 µg/ml), compared to identical cultures grown without gliotoxin (5 µg/ml). Grown using tryptic soy broth (TSB) media for 180 min in the log phase (0.3–0.4 OD600).
| Protein description | Fold Change (log2) | p-value | Peptides | Sequence coverage [%] | Protein IDs |
|---|---|---|---|---|---|
| 50S ribosomal protein L33 3 | Absent | N/A | 2 | 26.5 | P59628 |
| PTS system, beta-glucoside-specific IIABC component | Absent | N/A | 5 | 15.2 | Q831B4 |
| 6-aminohexanoate-cyclic-dimer hydrolase, putative | Absent | N/A | 10 | 19.1 | Q836S5 |
| Prephenate dehydrogenase | Absent | N/A | 7 | 23.4 | H7C6X1 |
| Mga domain-containing protein | Absent | N/A | 6 | 15.6 | Q82ZN7 |
| Protease synthase and sporulation negative regulatory protein pai 1 | Absent | N/A | 2 | 11.7 | Q82ZP9 |
| Transcriptional antiterminator, bglG family | Absent | N/A | 3 | 7 | Q82ZT1 |
| FolC family protein | Absent | N/A | 6 | 19.3 | Q82ZW9 |
| DNA polymerase III, delta prime subunit | Absent | N/A | 3 | 8.9 | Q830L8 |
| Acetyltransferase, GNAT family | Absent | N/A | 3 | 21.3 | Q830S1 |
| Diacylglycerol kinase catalytic domain protein | Absent | N/A | 3 | 12.9 | Q830V8 |
| ABC transporter, permease protein | Absent | N/A | 3 | 16.7 | Q831K7 |
| ComE operon protein 2, putative | Absent | N/A | 4 | 29.3 | Q831Q2 |
| Uncharacterized protein | Absent | N/A | 1 | 25.5 | Q831U5 |
| YxeA family protein | Absent | N/A | 3 | 26.5 | Q832L5 |
| tRNA-specific adenosine deaminase | Absent | N/A | 2 | 16.2 | Q832M0 |
| Probable dual-specificity RNA methyltransferase RlmN | Absent | N/A | 9 | 34.5 | Q833B6 |
| Putative 3-methyladenine DNA glycosylase | Absent | N/A | 4 | 19.2 | Q833H5 |
| YitT family protein | Absent | N/A | 5 | 21.2 | Q833I6 |
| Uncharacterized protein | Absent | N/A | 3 | 63.3 | Q833K1 |
| Glycerol uptake facilitator protein | Absent | N/A | 2 | 9.8 | Q833L8 |
| Phosphate transport system permease protein PstA | Absent | N/A | 2 | 7.1 | Q834B2 |
| Bacteriocin-protection, YdeI or OmpD-Associated | Absent | N/A | 2 | 10.5 | Q834C8 |
| Cold-shock domain family protein | Absent | N/A | 2 | 63.2 | Q834D5 |
| Metal-independent alpha-mannosidase | Absent | N/A | 10 | 33.8 | Q834E7 |
| Glyoxylase family protein | Absent | N/A | 5 | 18.8 | Q834I3 |
| V-type ATP synthase subunit D | Absent | N/A | 4 | 32.7 | Q834X7 |
| Exodeoxyribonuclease 7 small subunit | Absent | N/A | 2 | 46.1 | Q836W5 |
| Pyrroline-5-carboxylate reductase | Absent | N/A | 3 | 14.8 | Q836Y3 |
| 3-demethylubiquinone-9 3-methyltransferase | Absent | N/A | 2 | 12.1 | Q837B9 |
| UPF0178 protein EF_0842 | Absent | N/A | 5 | 45.3 | Q837J5 |
| DUF1189 domain-containing protein | Absent | N/A | 2 | 8.2 | Q838I5 |
| Putative N-acetylmannosamine-6-phosphate 2-epimerase | Absent | N/A | 3 | 18.1 | Q839T3 |
| 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase | Absent | N/A | 4 | 15.2 | Q839U9 |
| PTS system, IIB component* | Absent | N/A | 8 | 50 | Q82ZC7 |
*This protein exhibited overall decreased abundance > 1.5-fold and was only detectable in 1 of 3 treated samples.