Table 3.
Proteins which are uniquely absent in E. faecalis when grown in the presence of gliotoxin (5 µg/ml), compared to identical cultures grown without gliotoxin (5 µg/ml). Grown using tryptic soy broth (TSB) media for 30 min in the log phase (0.3–0.4 OD600).
| Protein description | Fold Change (log2) | p-value | Peptides | Sequence coverage [%] | Protein IDs |
|---|---|---|---|---|---|
| Endonuclea_NS_2 domain-containing protein | Absent | N/A | 2 | 17.4 | Q835Q6 |
| ABC transporter, ATP-binding/permease protein | Absent | N/A | 5 | 12.3 | Q837A1 |
| Uncharacterized protein | Absent | N/A | 3 | 41.7 | Q82YV6 |
| Lipase, putative | Absent | N/A | 4 | 15.1 | Q82Z80 |
| ATP-dependent DNA helicase RecG | Absent | N/A | 4 | 8.6 | Q82ZE7 |
| YitT family protein | Absent | N/A | 3 | 12.4 | Q82ZG6 |
| Beta-hydroxyacyl-ACP dehydratase | Absent | N/A | 2 | 27.3 | Q833N7 |
| Phosphate transport system permease protein PstA | Absent | N/A | 2 | 7.1 | Q834B2 |
| ABC transporter, ATP-binding protein | Absent | N/A | 3 | 23.2 | Q835Q5 |
| PTS system, IIA component | Absent | N/A | 3 | 50 | Q836T9 |
| DUF2200 domain-containing protein* | Absent | N/A | 4 | 49.1 | Q833J9 |
| YlbF family regulator* | Absent | N/A | 2 | 20.1 | Q831P6 |
*This protein exhibited overall decreased abundance > 1.5-fold and was only detectable in 1 of 3 treated samples.