Table 3.
Enzyme kinetics of α-glycosidase and α-amylase by 7d.
| Compound | Enzyme | Treatment | Mode of Inhibition x | Km (mM) | Vmax 103 (µM/min)−1 |
Ki (mg) y,z |
|---|---|---|---|---|---|---|
| 7d | α-glycosidase | Control | Competitive | 0.86 | 27.86 | 0.47 ± 0.35 |
| IC20 0.03 μg | 1.79 | 28.82 | ||||
| IC40 0.06 μg | 2.50 | 29.00 | ||||
| IC60 0.08 μg | 4.20 | 29.29 | ||||
| α-amylase | Control | Competitive | 0.44 | 13.00 | 0.75 ± 0.25 | |
| IC20 0.08 μg | 0.94 | 13.01 | ||||
| IC40 0.16 μg | 1.43 | 13.90 | ||||
| IC60 0.25 μg | 1.99 | 13.15 |
x Inhibition mode was determined from the Lineweaver–Burk plot; y Ki = dissociation constant; z Values are expressed as mean ± SE.