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. 2023 Sep 16;11(9):2331. doi: 10.3390/microorganisms11092331

Table 2.

LC–MS gradient elution procedure.

Time (min) Water (%) Acetonitrile (%)
0 77 23
13 54 46
33 32 68
45 32 68
55 0 100
60 0 100
63 77 23