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. 2023 Sep 5;12(18):3179. doi: 10.3390/plants12183179

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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RT-qPCR analysis of the expression level of eight candidate genes in different samples. (A) TRINITY_DN81698_c0_g1, (B) TRINITY_DN156398_c0_g1, (C) TRINITY_DN22597_c0_g2, (D) TRINITY_DN40863_c0_g1, (E) TRINITY_DN55799_c0_g1, (F) TRINITY_DN4024_c0_g1, (G) TRINITY_DN74597_c0_g1, and (H) TRINITY_DN17585_c0_g1. The relative expression changes of the target gene in NM, CG1, and CG2 treatments were calculated by the 2^−∆∆Ct method, and are shown as means ± SE (n = 3). The statistically significant difference between NM and CG1/CG2 treatments is tested by Student’s t-test (* p < 0.05; ** p < 0.01; *** p < 0.001, ns indicates no significant difference).