Figure 5.

Viral loads in the lungs of mice infected three months after boosting. Four to six days after the challenge, mice (6 per group) were sacrificed, and the lungs were processed for the extraction of total RNA. Then, 1 μg of total RNA from each infected mouse was analyzed by RT-qPCR for the presence of both SARS-CoV-2 E- and N-specific RNAs. Shown are the numbers of E- and N-specific viral RNA copies amplified from total RNA isolated from the lungs of each animal, together with intragroup mean values ±SE. Dotted lines signal the detection limits of the assay.