Table 2.
Genotoxicity tests in bacteria (TA98 and TA 100) of EECR95.
Groups | Colony Forming Units (CFU) | |
---|---|---|
−S9 1 | +S9 | |
TA98 | ||
Blank 2 | 39 ± 4.4 a | 44 ± 4.8 a3 |
Positive control | 468 ± 4.5 c (4-NQNO) 2 | 518 ± 8.5 c (2-AF) |
0.25 mg/plate | 42 ± 4.2 a | 45 ± 3.6 a |
0.5 mg/plate | 44 ± 3.3 a | 49 ± 4.1 a |
1.0 mg/plate | 49 ± 6.3 a | 51 ± 5.1 a |
TA100 | ||
Blank | 44 ± 7.1 a | 47 ± 7.3 a |
Positive control | 428 ± 8.2 a | 475 ± 7.4 a3 |
0.25 mg/plate | 46 ± 5.4 a | 48 ± 5.9 a |
0.5 mg/plate | 51 ± 6.7 a | 52 ± 5.5 ab |
1.0 mg/plate | 51 ± 6.9 a | 51 ± 6.6 a |
1 S9: 9000× g supernatant in liver homogenate. 2 Blank: treated with PBS. Controls were treated with 4-nitroquinoline-N-oxide (4-NQNO; direct mutagenic test) or 2-aminoanthracene (2-AF; indirect mutagenic test). 3 Values (means ± SD, n = 3) in each column not sharing the same superscript letter are significantly different (p < 0.05).