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. 2023 Aug 28;19(14):4657–4671. doi: 10.7150/ijbs.85767

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PHB2 overexpression sustains cardiomyocyte viability and mitochondrial function during LPS exposure. Cultured HL-1 cells transfected with PHB2 adenovirus (Ad/PHB2) or control adenovirus (Ad/Ctrl) were treated with LPS to simulate endotoxemia-mediated cardiac dysfunction in vitro. (A) Analysis of cell viability (CCK-8 assay). (B, C) Analysis of apoptosis by PI staining. (D, E) Evaluation of mitochondrial membrane potential in cells loaded with the JC-1 probe. (F-H) ELISA-based analysis of Gpx4, Prx3, and Txnrd2 protein concentrations in HL-1 cells. (I, J) ELISA-based analysis of Bcl-2 and Bax protein concentrations in HL-1 cells. #p<0.05 vs. PBS+WT group; *p<0.05 vs. LPS+WT group.