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. 2023 Aug 28;19(14):4657–4671. doi: 10.7150/ijbs.85767

Figure 3.

Figure 3

PHB2 phosphorylation maintains mitochondrial localization of PHB2 and promotes mitophagy and UPRmt. (A, B) Western blot analysis of PHB2-Ser39 phosphorylation in HL-1 cells. #p<0.05 vs. PBS+WT group. (C-E) Western blot analysis of mitochondrial and cytoplasmic PHB2 expression in HL-1 cells transfected with a phosphomimetic PHB2 mutant protein (PHB2S39D). #p<0.05 vs. PBS+Vector group; *p<0.05 vs. LPS+Vector group. (F-H) RT-qPCR analysis of transcriptional levels of the mitophagy-related markers Beclin1, FUNDC1, and Parkin in HL-1 cells. #p<0.05 vs. PBS+Vector group; *p<0.05 vs. LPS+Vector group. (I-L) RT-qPCR analysis of transcriptional levels of the UPRmt-related markers mtDnaJ, ClpP, LonP1, and Hsp10 in HL-1 cells. #p<0.05 vs. PBS+Vector group; *p<0.05 vs. LPS+Vector group.