Figure 4.
LPS induces Pgam5-dependent PHB2 dephosphorylation. Cultured HL-1 cells were transfected with shRNA against Pgam5 (sh/Pgam5) or control shRNA (sh/Ctrl) prior to exposure to LPS. (A, B) Western blot analysis of PHB2 phosphorylation in HL-1 cells. (C, D) Molecular docking analysis of the Pgam5-PHB2 interaction. The potential amino acid sites required for the binding between PHB2 and Pgam5 were analyzed. #p<0.05 vs PBS+sh/Ctrl group; *p<0.05 vs. LPS+sh/Ctrl group.
