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. 2023 Aug 28;19(14):4657–4671. doi: 10.7150/ijbs.85767

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PHB2S39A transfection abolishes the protective effects of Pgam5 deletion on cardiomyocyte viability and mitochondrial function. (A) Cell viability analysis (CCK-8 assay) in HL-1 cardiomyocytes. (B, C) Analysis of apoptosis by PI staining. (D, E) Evaluation of mitochondrial membrane potential in HL-1 cells loaded with JC-1. (F-H) ELISA-based analysis of Gpx4, Prx3, and Txnrd2 protein concentrations. (I, J) ELISA-based analysis of Bcl-2 and Bax concentrations. #p<0.05 vs PBS+sh/Ctrl group; *p<0.05 vs. LPS+sh/Ctrl group; @p<0.05 vs. LPS+sh/Pgam5 group.