Figure 2.

Chitinase activity in the culture supernatant of Microbulbifer thermotolerans, Vibrio spp., Aquimarina muelleri, and Zobellia galactinovorans. Reactions were carried out for 15 min, at 37 °C, using colloidal chitin (0.1%) as a substrate, and 20 mM sodium phosphate buffer, pH 6.0. The experiments were performed in triplicate and standard deviations are shown as error bars.