Figure 4.

Accumulation of HSV-1 DNA and representative viral transcripts following PKR silencing. HEp-2 cells were transfected with si-PKR#2 (10 µM) and infected with HSV-1 (10 MOI). Scrambled siRNA was used as no-target control. The samples were collected 24 h post-infection (a) viral DNA, and (b) viral transcripts were analyzed. The changes levels of ICPO, UL42, and US11 genes were analyzed by calculating the value of 2^−ΔΔCt. The assay was performed as means of triplicate ± SD and expressed as fold change over the housekeeping genes. *** < p <0.0001 and ** <0.01.