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Mutational analysis of segment D. (A) Change of conserved (bold) amino acids in segment D by mutations M1–M4. (B) The Gal4–SV40 enhancer/promoter–luciferase reporter was transfected alone (–) or co-transfected with plasmids encoding Gal4–TRD-wt or Gal4–TRD mutants M1–M4 into HEK293 cells. Results are representative of at least four separate experiments, each in triplicate (± SD).
