Figure 2.

The anti-hP2X7 mAb prevents the loss of hTregs and hNK T cells in vitro. (A) Illustration of the in vitro cultures. (B–I) hPBMCs (n = 6 donors) were cultured overnight (20 h) in RPMI-1640 medium containing 2.5% FCS in the presence of 2 µg/mL of the isotype control or anti-hP2X7 mAb. The proportions of (B) hCD3⁺ T cells, (C) hCD4⁺ and (D) hCD8⁺ T cell subsets, (F) hCD4⁺hCD25⁺hCD127^lo hTregs, (G) hCD56⁺ hCD3⁺ hNK T cells, (H) hCD56⁺ hCD3⁻ hNK cells, or (I) CD19⁺ B cells were determined by flow cytometry. (E) The ratio of hCD4⁺ to hCD8⁺ T cells was calculated from (C,D). (B–I) Data represented as mean ± SEM, symbols represent individual donors. Significance was assessed by the paired Student’s t-test, with p values as shown.