Figure 5.

The anti-hP2X7 mAb increases proportions of splenic hTregs and hNK cells in humanised mice at Day 21. (A–L) Spleens from mice treated with the isotype control (n = 8) or anti-hP2X7 (n = 8) mAb were collected at Day 21 (or humane endpoint) and immune cell subsets were analysed by flow cytometry. Proportions of (A) hCD45⁺ leukocytes were first identified before determining the proportions of (B) hCD3⁺ T cells, (C) hCD4⁺ and hCD8⁺ T cells, (E) hCD4⁺hCD25⁺hCD127^lo hTregs, (F) hCD39⁺ hTregs, (G) hCD4⁺hCD161⁺hCD39⁺ hTh17 cells, (I) hCD8⁺hCD161^high Tc17 cells, (J) hCD3⁺hCD56⁺ hNK T cells, (K) hCD3⁻hCD56⁺ hNK cells, and (L) hCD19⁺ B cells. (D) The hCD4⁺:hCD8⁺ T cell ratio was calculated from (C). (H) The hTh17:hTreg ratio was calculated from (E,G). (A–L) Data represented as the mean ± SEM. Symbols represent individual mice. Significance was assessed by the (A,B,E–H) unpaired Student’s t-test, (C) one-way ANOVA, or (D,I–L) Mann–Whitney test, with p values as shown.