Figure 8.

The anti-hP2X7 mAb altered cell proportions in the spleen of humanised mice at humane or experimental (Day 75) endpoint. (A–M) Spleens from mice treated with isotype control (n = 7–14) or anti-hP2X7 (n = 7–13) mAb were collected at humane or experimental (Day 75) endpoint and immune cell subsets were analysed by flow cytometry. Proportions of (A) hCD45⁺ leukocytes were first identified before determining proportions of (B) hCD3⁺ T cells, (C) hCD4⁺ and hCD8⁺ T cells, (E) hCD4⁺hCD25⁺hCD127^lo hTregs, (F) hCD39⁺ hTregs, (G) hCD4⁺hCD161⁺hCD39⁺ hTh17 cells, (I) hCD8⁺hCD161^high hTc17 cells, (J) hCD3⁺hCD56⁺ hNK T cells, (K) hCD3⁺ hVα24-Jα18⁺ hiNK T cells, (L) hCD3⁻hCD56⁺ hNK cells and (M) hCD19⁺ B cells. (D) The hCD4⁺:hCD8⁺ T cell ratio was calculated from (C). (H) The hTh17:hTreg ratio was calculated from (E,G). (A–M) Data represented as mean ± SEM. Symbols represent individual mice. Significance was assessed by the (A,E,G) unpaired Student’s t-test, (B,D,F,H–M) Mann-Whitney test, or (C) one-way ANOVA, with p values as shown.