Table 2.
Limit of detection analyses on serially diluted DNA in triplicate.
| Sample | Copies/µL | Copies/Reaction | PrRT Positive Reactions | INT Positive Reactions |
|---|---|---|---|---|
| 8E5 cell line DNA | 515.8 | 5157.6 | 3 | 3 |
| 103.2 | 1031.5 | 3 | 3 | |
| 51.6 | 515.8 | 3 | 3 | |
| 25.8 | 257.9 | 3 | 3 | |
| 12.9 | 128.9 | 3 | 3 | |
| 6.4 | 64.5 | 3 | 3 | |
| 3.2 | 32.2 | 3 | 3 | |
| 1.6 | 16.1 | 2 * | 2 * | |
| 0.8 | 8.1 | 3 | 3 | |
|
Study
sample |
12.7 | 127.0 | 3 | 3 |
| 6.35 | 63.5 | 2 * | 3 | |
| 3.18 | 31.8 | 3 | 3 | |
| 1.59 | 15.9 | 3 | 3 | |
| 0.79 | 7.9 | 3 | 3 | |
| 0.4 | 4.0 | 3 | 3 | |
| 0.2 | 2.0 | 0 | 1 |
* Serial dilutions at low DNA concentrations risk exclusion of target DNA from some aliquots.