Table 3.

Human and HIV-1 DNA copy numbers required for positive target amplification.

Sample Type	Sample ID	Copies/µL		Targeted HIV-1 PCR Positive
		Human DNA	HIV-1 DNA	PrRT	INT
Adults	ID_37	24,112.4	0.46	Positive	Positive
	ID_32	202,714.3	0.65	Positive	Positive
	ID_33	165,062.9	2.01	Positive	Positive
	ID_36	163,217.1	2.27	Positive	Positive
	ID_30	297,115.4	4.16	Positive	Positive
	ID_34	117,258.8	6.72	Positive	Positive
	ID_35	162,380.1	8.05	Positive	Positive
	ID_38	320,693.1	10.42	Positive	Positive
	ID_29	172,073.2	12.7	Positive	Positive
Children	ID_C_16	10,561.8	<0.1 *	Negative	Positive
	ID_C_25	18,334.3	<0.1 *	Positive	Positive
	ID_C_40	31,856.5	<0.1 *	Positive	Positive
	ID_C_32	23,190.2	0.1	Positive	Positive
	ID_C_28	19,819.5	0.2	Positive	Positive
	ID_C_4	26,576.4	0.2	Positive	Positive
	ID_C_29	20,857.2	0.3	Negative	Positive
	ID_C_2	20,314.4	0.5	Positive	Positive
	ID_C_31	12,519.1	0.7	Positive	Positive
	ID_C_17	9308.4	0.8	Positive	Positive
	ID_C_38	16,371.8	1.1	Positive	Positive
	ID_C_24	12,138.6	1.6	Positive	Positive
	ID_C_33	20,367.2	1.7	Negative	Positive
	ID_C_15	22,215	1.9	Positive	Positive
	ID_C_9	17,076.1	12	Negative	Negative

* Less than 0.1 c/µL of HIV-1 DNA detected in quantification results equates to a single copy per reaction.