D-DPTIP does not affect tau and pTau expression levels in PS19 mice. (A) Representative immunoblotting images from hippocampal tissue of male mice showing total tau (upper blot) and pTau (lower blot). GAPDH was used as a loading control. WT+ vehicle treatment (N = 6), PS19 + vehicle treatment (N = 5), PS19 + D-DPTIP treatment (N = 6) were utilized. (B) Quantification of A for total tau normalized by total protein. (C) Quantification of A for pTau normalized by total protein. (D) Representative immunoblotting images from hippocampal tissue of female mice showing total tau (upper blot) and pTau (lower blot); GAPDH used as a loading control. WT+ vehicle treatment (N = 6), PS19 + vehicle treatment (N = 6), PS19 + D-DPTIP treatment (N = 6) were utilized. (E) Quantification of D for total tau normalized by total protein. (F) Quantification of D for pTau normalized by total protein. Statistics were performed using unpaired Student’s t-tests in (B,C,E,F). Bars represent mean ± SEM.