Table 4.
Parametric grading table of allogeneic finished products after 6 h of transport and storage. AB, Alcian Blue; ACAN, aggrecan; DMMB, dimethylmethylene blue; GAG, glycosaminoglycan; HA, hyaluronic acid; HE, hematoxylin and eosin; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NaCl, sodium chloride; PBS, phosphate-buffered saline.
| Parameter | Controls | Targets/Acceptance Criteria | Endpoint Construct Grading 1 | ||
|---|---|---|---|---|---|
| NaCl | OPTIMEM | HA-PBS | |||
| Cellular Viability | MTT | Presence of viable cells on the constructs | +++ | +++ | +++ |
| Cellular Repartition | MTT/HE | Homogeneous viable cell repartition on one side of the scaffold | +++ | +++ | +++ |
| Construct Morphology | Operator Assessment | Specific macroscopic change in construct color and rigidity | +++ | +++ | +++ |
| GAG Content | DMMB | Maintenance of total GAG contents | ++ | +++ | +++ |
| Aggrecan Presence | Histology | Maintenance of positive ACAN staining on one side of the construct | +++ | +++ | +++ |
| Alcian Blue Staining | Histology | Maintenance of positive AB staining on one side of the construct | +++ | +++ | +++ |
1 Semi-quantitative grading was performed using the abbreviated nomenclature presented hereafter. (++) = satisfactory; (+++) = optimal.