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Dimethyl sulfoxide–treated U2OS cells treated with the Cell Painting assay; the six dyes in five channels stain eight cellular compartments. Top row (left to right): mitochondrial staining; actin, Golgi, and plasma membrane staining; and nucleolar and cytoplasmic RNA staining. Bottom row (left to right): endoplasmic reticulum staining, DNA staining, a montage of all five channels.
