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A) Assessment of decreasing or increasing the number of cells plated by 20% on ability to perform Percent Matching. Few consistent effects are seen. B) Assessment of using parental cell lines or Cas9 expressing polyclonal lines for compound treatments. Few consistent effects are seen; ability to match compounds in parental vs Cas9 cells against CRISPR (required to be performed in Cas9 cells) does not seem extremely different at either common time point.
