Extended Data Figure 3-.

Assessment of the effect of gene-treatment-related compounds on image-based profiling. A) Addition of blasticidin to ORF plates or puromycin to CRISPR plates does not appear to improve percent matching across modalities vs unselected plates. B) Addition of selection compounds may have a deleterious effect on percent matching vs unselected plates, though we cannot rule out that this is due to fewer replicates for the selected plates than the unselected ones. C) Addition of 4μg/mL polybrene for 24 hours may produce a phenotypic effect; polybrene addition displays decreased inter-treatment cross-plate percent replicating vs intra-treatment cross-plate-percent replicating, even though both sets of plates were part of the same batch. D) Addition of polybrene to Target2 treated cells does not improve percent matching between Target2 treated plates and ORF treated plates from a previous batch.