Table 1.
Primer sequences used for HPgV-1 genotyping by nested RT-PCR (adapted from Naito; Abe 2001).
| Primer | Sequence a (Specificity/Polarity) |
Isolate Designed for Primer Sequences b |
|---|---|---|
| HG1 | 5′-GGTCGTAAATCCC GGTCACC-3′ (universal/sense) |
PNF2161 (U44402; 139 to 158) |
| HG1R | 5′-CCCACTGGTCCTT GTCAACT-3′ (universal/antisense) |
PNF2161 (U44402; 381 to 400) |
| HG2R | 5′-ATTGAAGGGCGA CGTGGACC-3′ (universal/antisense) |
PNF2161 (U44402; 331 to 350) |
| Mix A | ||
| G38 | 5′-TGTAATAAGGACC CGGCGMT-3′ | GBV-C (U36380; 239 (type to 257) 1-specific/sense) |
| G41 | 5′-TGGTCAAGGTCCC TCTG-3′ | HGV-VT2 (AB013193; (type 28 to 44) 3-specific/sense) |
| Mix B | ||
| G35 | 5′-GGGTCTTAAGAG AAGGTTAAGA-3′ |
PNF2161 (U44402; 177 to 198) (type 2-specific/sense) |
| G40 | 5′-GGGTYAAGGCAC CTCTTA-3′ |
HGV-MY14 (type (AB021287; 28 to 45) 4-specific/sense) |
a M = A or C, Y = C or T. b Numbers in parentheses indicate database accession number and nucleotide positions.