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Localization and inducibility of protein–DNA complexes formed with the NF-κB 2B1/2 element. Extracts were prepared from rat liver nuclei (lanes 1–7) and cytoplasm (lanes 8–14) and tested for complex formation using the NF-κB 2B1/2 element as probe. Inducibility of complex formation was tested by the addition of DOC, formamide or NP-40, as indicated. The C1 complex (lanes 1–7) was restricted to nuclear extracts and was unaffected by treatment with dissociating reagents.
