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. 2000 May 15;28(10):2091–2098. doi: 10.1093/nar/28.10.2091

Figure 4.

Figure 4

Supershift assays using antibodies against NF-κB subunits p50, p52 and p65 and against RBP-Jκ. Band shift reactions contain HeLa cell nuclear extract (N.E., lanes 1–10) and a kinase-labeled NF-κB 2B1/2 probe. Reactions were carried out in the absence (lanes 1–5) or presence (lanes 6–14) of DOC and NP-40. Lanes 11–14 contain antibody alone. The results indicate that Ab-p50 (lanes 2 and 7) and Ab-p65 (lanes 3 and 8) supershift the upper complex, while Ab-RBP-Jκ (lanes 5 and 10) supershifts the lower complex. The positions of RBP-Jκ, NF-κB and supershifted complexes are indicated to the left.