Figure 7.

Formation of NF-κB, RBP-Jκ and several other protein–DNA complexes does not differ in rat hepatic extracts prepared from control (C) or PB-induced animals (I). Oligonucleotides spanning the CYP2B1/2 core (–45 to –13) (lanes 1 and 2) and the AdML TATA element (lanes 3 and 4) were used to normalize activity of control (C) and PB-induced (I) extracts with respect to TATA element binding activities. Oligos tested include NF-κB Igκ (lanes 5 and 6), NF-κB 2B1/2 (lanes 7 and 8), AP-1 (lanes 9 and 10), Sp1 (lanes 11 and 12) and C/EBP (lanes 13 and 14).