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. 2023 Sep 12;15(9):1909. doi: 10.3390/v15091909

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Viral shedding (buccal and cloacal) from chickens directly infected (D0) with (a) H5N8-2020 (A/chicken/England/030786/2020) or (b) H5N1-2020 (A/mute swan/England/SA14-234255/2020) at low, medium, and high doses. Continual lines and symbols indicate mean and individual shedding, respectively, in each group. Shedding from directly infected (D0), first-contact (R1), and second-contact (R2) groups are shown with black, red, and green lines/symbols, respectively. DPI indicates days post-infection, measured relative to the initial D0 inoculation day. Tables on the right summarise the proportions (%) of infected chickens in each group for H5N8-2020 (upper) and H5N1-2020 (lower), with ND (“not done”) indicating where R2 chickens were not introduced because there was no prior transmission to the R1 stage.