Table 1.

Properties of the 2-methyltetrol, 2-methyltetrol sulfate and 3-methyltetrol sulfate standards characterized by HILIC/ESI-HR-Q-TOFMS, including retention times (RTs), linear range (L. range), coefficient of determination (R²), limit of detection (LOD), limit of quantification (LOQ) of ten replicate injections. Note that structures are for one of two diastereomers for each standard and ions are shown for the methyltetrol sulfates

Standard	Synthesized structural isomer	Structure	[M − H]−	m/z	RT(s) (min)	L. range (μg mL−1)	R 2	LOD (μg L−1)	LOQ (μg L−1)
2-Methyltetrols	2-Methylerythritol and 2-methylthreitol		C5H11O4−	135.066	4.0	0.01–25	0.9994	7.74	25.8
2-Methyltetrol sulfates	1,3,4-Trihydroxy-2-methylbutan-2-yl sulfate		C5H11O7S−	215.023	2.1, 2.6, 4.2, 5.2	0.01–10	0.9996	1.72	5.75
3-Methyltetrol sulfates	2,3,4-Trihydroxy-3- methylbutyl sulfate		C5H11O7S−	215.023	2.1, 2.6, 4.2, 5.2, 8.0, 8.3	0.01–25	1.0000	3.83	12.8