Table 7.
Heterologous expression of proteases.
| Host strains for cloning and overexpression | Proteases or encoding genes | Plasmid vectors | References |
|---|---|---|---|
| B. subtilis | |||
| B. subtilis | Glutamyl endopeptidase from Bacillus intermedius (gseBi) | pV | Shagimardanova et al. (2007) |
| B. subtilis DB104 | A thermophilic neutral protease from Bacillus stearothermophilus | Shuttle vector pHP13 | Zhang M. et al. (2008) |
| B. subtilis | An alkaline serine protease gene (GsProS8) from Geobacillus stearothermophilus | pWB980 | Chang et al. (2021) |
| B. subtilis WB800 | A gene coding for the nattokinase (Nk) from B. subtilis strain VTCC-DVN-12-01 | pAC7 | Nguyen et al. (2013) |
| B. subtilis WB800 | A subtilisin-like alkaline serine protease (ASP) from Bacillus halodurans C-125 | pMA0911 | Tekin et al. (2020) |
| B. subtilis WB600 | Keratinase (kerT) gene | pLY | Cao et al. (2019) |
| B. subtilis WB600 | Alkaline serine protease (BcaPRO) from B. clausii | pWBPRO1 constructed based on pWB980 | Liu et al. (2019) |
| B. subtilis SCK6 | A novel streptomyces trypsin | pWB980 | Wang et al. (2019) |
| A recombinant B. subtilis | Extracellular thermostable alkaline halophilic protease | pSaltExSePR5 | Promchai et al. (2018) |
| B. subtilis | Alanine aminopeptidase from Bacillus licheniformis E7 | pMA0911 | Chen Y. et al. (2022) |
| B. subtilis SCK6 | An extracellular keratinase | pMA0911 | Tian et al. (2019) |
| A recombinant B. subtilis | Serine protease from Bacillus intermedius | pCB22 | Sharipova et al. (2008) |
| B. subtilis | A fibrinolytic enzyme (subtilisin DFE) gene | pSUGV4 | Peng et al. (2004) |
| B. subtilis WB600 | Fibrinolytic protease of Bacillus licheniformis CH 3–17 | pHY300PLK | Jo et al. (2011) |
| E. coli | |||
| E. coli | An intracellular serine protease from isolated salt-tolerant Bacillus sp. LCB10 | pET-30a | Hou et al. (2019) |
| E. coli Transetta (DE3) | Subtilisin-like protease from a thermophilic Thermus thermophilus HB8 | pET-22b (+) | Xie et al. (2019) |
| E. coli strain BL21 | Serine alkaline protease | pET-15b | Suberu et al. (2019) |
| E. coli BL21-Gold (Stratagene), E. coli ORIGAMI B and E. coli Rosetta2 | Extracellular serine proteases from Stenotrophomonas maltophilia | pMS470Δ8 | Ribitsch et al. (2012) |
| E. coli BL21(DE3) pLysS and E. coli BL21-AI™ | Serine alkaline protease (SAPN) from Melghiribacillus genus. | pUT57 and pTrc99A, Gateway™ pDEST™ 17 | Mechri et al. (2021) |
| E. coli BL21 | A novel alkaline serine protease gene from native Iranian Bacillus sp. | pET-28 a (+) | Ariyaei et al. (2019) |
| E. coli | Serine protease from Nocardiopsis sp. | pET-39b (+) and pET-22b (+) | Rohamare et al. (2015) |
| E. coli BL21 (DE3) | A thermo- and surfactant-stable protease from Thermomonospora curvata | pET-25b (+) | Sittipol et al. (2019) |
| E. coli BL21 (DE3) | Serine proteases from Oceanobacillus iheyensis O.M.A18 and Haloalkaliphilic bacterium O.M.E12 | pET-21a (+) | Purohit and Singh (2014) |
| E. coli BL21 (DE3) | Subtilisin-like Protease Myroicolsin | pET-22b (+) | Ran et al. (2014) |
| E. coli BL21 | An alkaline protease from Bacillus licheniformis | pET–28b (+) | Lin et al. (2015) |
| E. coli BL21(DE3) | A fibrinolytic protease gene from the polychaeta, Periserrula leucophryna | pT7-7 | Joo et al. (2013) |
| E. coli BL21(DE3) | A novel fibrinolytic serine protease from Arenicola cristata | pET-21a (+) | Zhao and Ju (2014) |
| E. coli BL21(DE3) | A novel aspartic protease gene from marine-derived Metschnikowia reukaufii | pET-24a (+) | Li et al. (2008) |
| E. coli BL21(DE3) | A novel extracellular subtilisin-like protease from the hyperthermophile Aeropyrum pernix K1 | pGEX | Catara et al. (2003) |
| E. coli BL21(DE3) | A new thiol-dependent, alkaline serine protease | pET-28a (+) | Masilamani and Natarajan (2015) |
| E. coli BL21(DE3) pLysS | A serine protease-like protein from silkworm (Bombyx mori) | pGEX-5X-1 | Kim et al. (2009) |
| E. coli BL21 | Lon protease from rice (Oryza sativa) | pET-32a | Su et al. (2006) |
| E. coli Rosetta-gami (DE3) | Serine protease aprv2 from virulent isolate Dichelobacter nodosus | pET-22b (+) | Wani et al. (2016) |
| E. coli BL21 (DE3) | A novel extracellular cold-adapted alkaline protease gene of the marine bacterium strain YS-80-122 | pET-28a | Wang et al. (2010) |
| E. coli Shuffle®T7. | Three main serine carboxypeptidases (SCP3, SCP20, and SCP47) from Nepenthes mirabilis | pET-28a-SUMO | Porfírio et al. (2022) |
| E. coli BL21(DE3) | Carboxypeptidases genes (dacA, dacB, dacC, and dacF) in Bacillus subtilis CW14 | pET-28a (+) | Xu et al. (2021) |
| E. coli | A collagenolytic aspartic protease from Thermomucor indicae-seudaticae | pET-28a (+) | Pereira et al. (2020) |
| E. coli BL21(DE3) pLysS | Novel protease from Bacillus licheniformis strain K7A | pGEM-T Easy | Hadjidj et al. (2018) |
| E. coli BL21 (DE3) | A low salt-adapted extracellular protease from the extremely halophilic archaeon Halococcus salifodinae | pET-28a | Hou et al. (2021) |
| E. coli BL21 | Avihepatovirus 3C protease | pET-32a | Sun et al. (2019) |
| E. coli BL21 (DE3) pLysS | Site-2 protease | pHEN6 | Schacherl et al. (2017) |
| E. coli BL21 (DE3) | A dual-functional aminopeptidase from Streptomyces canus T20 | pET-24a (+) | Qin et al. (2021) |
| P. pastoris | |||
| P. pastoris SMD1168 and X33 | Serine alkaline protease from Melghiribacillus genus | pPICZαC | Mechri et al. (2021) |
| P. pastoris GS115 | Serine protease from thermophilic fungus Thermoascus aurantiacus var. levisporus | pPIC9K | Li et al. (2011) |
| Saccharomyces cerevisiae W3124 | An aspartic protease | pYES 2.0 | Bang et al. (1999) |
| P. pastoris | Proteinase K | pPink-HC | Skowron et al. (2020) |
| P. pastoris KM71 | Protease from Aspergillus niger | pPIC9K | Ke et al. (2019) |
| P. pastoris | A plant aspartic protease (preprogaline B) | pGAPZα A | Feijoo-Siota et al. (2018) |
| P. pastoris | A novel serine protease | A self-construct plasmid | Shu et al. (2016) |
| P. pastoris X-33 and GS115 (his4) | Streptokinase | pPICZαA and pGAPZαA | Adivitiya Dagar et al. (2016) |
| P. pastoris | Bacillus pumilus 3–19 protease | pPINK-HC | Pudova et al. (2021) |
| P. pastoris | A zinc-dependent proteases of the metzincin superfamily of metalloproteases | pPIC9K | Schlenzig and Schilling (2017) |
| P. pastoris GS115 | Recombinant protease MarP from Mycobacterium tuberculosis | pPICZα | García-González et al. (2021) |
| P. pastoris | A collagenolytic aspartic protease from Thermomucor indicae-seudaticae | pPICZαA | Pereira et al. (2020) |
| P. pastoris | A novel aminopeptidase B from Aspergillus niger | pPIC9K | Song and Feng (2021) |
| P. pastoris X-33 and SMD1168 strains | A Mucor circinelloides aspartic protease | pGAM1 | Kangwa et al. (2018) |
| P. pastoris | A new serine protease from Crotalus durissus collilineatus venom | PICZαA | Boldrini-França et al. (2015) |
| P. pastoris | Keratinolytic serine protease gene sfp2 from Streptomyces fradiae var. k11 | pPIC9K | Li et al. (2007) |
| P. pastoris KM71 | Subtilisin Pr1A gene from a strain of locust specific fungus, Metarhizium anisopliae | pPIC9K | Zhang W. et al. (2008) |
| P. pastoris GS115 | Subtilisin QK | pPICZα | Zhou et al. (2017) |
| P. pastoris SMD1168 and X33 | A serine alkaline protease from Melghiribacillus thermohalophilus | pPICZαC | Mechri et al. (2021) |
| P. pastoris KM71 | Aspergillus sojae alkaline protease | pPIC9K | Ke et al. (2018) |
| P. pastoris GS115 | Recombinant Aspergillus oryzae alkaline protease | pPIC9K | Guo and Ma (2008) |
| P. pastoris | Keratinase (kerA) gene from Bacillus licheniformis | pPICZαA | Porres et al. (2002) |
| P. pastoris | A thermostable serine protease (TfpA) from Thermomonospora fusca YX | pPICZαA | Kim and Lei (2005) |
| P. pastoris GS115 | The Bacillus subtilis subsp. subtilis str. BSP1 YwaD aminopeptidase | pHBM905A | Tang et al. (2016) |
| P. pastoris GS115 | Carboxypeptidase Y from Saccharomyces cerevisiae | pHBM905A | Yu et al. (2014) |
| P. pastoris X-33 | Prolyl aminopeptidase | pPIC9K | Yang et al. (2016) |
| P. pastoris X-33 | X-Prolyl-dipeptidyl aminopeptidase from Basidiomycete ustilago maydis | pPICZαB | Juárez-Montiel et al. (2014) |
| P. pastoris X33 or SMD1168H | A metalloprotease | pPICZαC | Schlenzig et al. (2015) |
| P. pastoris GS 115 | A new dipeptidyl-peptidase isolated from Aspergillus fumigatus | pHIL-S1 | Beauvais et al. (1997) |
| P. pastoris GS115 | A metalloprotease | pPIC9K | Song et al. (2021b) |
| P. pastoris GS115 | A new carboxypeptidase from Aspergillus niger | pPIC9K | Song et al. (2021a) |
| P. pastoris GS115 | Two new Aspergillus niger aspartic proteases | pPIC9K | Song Y. et al. (2020) |
| Pichia pastoris KM71H | Serine protease from Bothrops pauloensis snake venom | pPICZαA | Isabel et al. (2016) |
| P. pastoris GS115 | A thermolysin-like protease, neutral protease I, from Aspergillus oryzae | pHBM905BDM | Ma et al. (2016) |
| P. pastoris GS115 | First fibrinolytic enzyme from mushroom (Cordyceps militaris) | pPIC9K | Katrolia et al. (2019) |
| P. pastoris GS115 | Thrombolytic enzyme (lumbrokinase) from earthworm | pPICZα-B | Ge et al. (2005) |