Skip to main content
. 2023 Aug 25;11(9):1420. doi: 10.3390/vaccines11091420

Figure 3.

Figure 3

Cellular response in BALB/c mice immunized with the mixture of A29L, M1R, B6R, and A35R of MPXV. A29L, M1R, B6R, and A35R in combination were formulated with or without adjuvant and used to immunize mice intramuscularly. At day 90, splenocytes were isolated and stimulated a with mixture of A29L, M1R, B6R, and A35R, and T cell immune response was analyzed via flow cytometry analysis and ELISPOT assays. (A–C) IFN-γ, TNF-α, and IL-4 of CD4+ T cells were analyzed via flow cytometry assay. (D–F) IFN-γ, TNF-α, and IL-4 of CD8+ T cells were analyzed via flow cytometry assay. (G) IFN -γ-producing T cells were analyzed via ELISPOT assays. Data are presented as means ± SEM (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, no significance (p > 0.05).