Fig 2.

Clade 2 RBD sarbecoviruses do not use any known coronavirus receptors for cell entry. (A) BHK-21 cells were transfected with human orthologs of known coronavirus receptors and then infected with viral isolates. Replication was quantified by qRT-PCR. (B) VSV-based pseudotyped virus particles bearing chimeric SARS-CoV spikes with the indicated virus RBDs were generated in HEK 293T cells and concentrated in OptiPrep. Spike was detected in cell lysates and pseudotyped virus particles by probing for FLAG. (C) Huh-7 cells or cells transduced to express human ACE2 were infected with pseudotyped virus particles, and luciferase was measured as a readout for cell entry. (D) BHK-21 cells were transfected with the indicated bat ACE2 alleles and infected with viral isolates. Replication was monitored by qRT-PCR.