Fig 2.

Disease manifestation changes from acute hepatitis to late-onset encephalitis in C57BL/6 mice infected with RVFVmiR-122. (A) C57BL/6 mice were monitored for survival after footpad infection with either RVFVmiR-184 (n = 6) or RVFVmiR-122 (n = 8). (B) Viral RNA loads of L or S segment of RVFV in the liver, spleen, and brain tissues from mice at the time of terminal euthanasia. Open circle in red represents one mouse that survived until the end of the experiment (21 dpi). Dotted line indicates LOD of qRT-PCR. (C) Representatives of liver and brain tissue staining by H&E or IHC probing for RVFV N protein imaged with 10× objective. Insets of indicated areas were imaged with 20× objective. (D) Blood from a subset of mice infected with either RVFVmiR-184 (n = 5) or RVFVmiR-122 (n = 5) were used for chemistry analysis at the time of euthanasia. ALT, alanine transaminase; GGT, gamma-glutamyl transferase; BA, bile acid. Dotted line represents LOD and gray area represents normal range of that test. Levels of GGT and BA in uninfected animals are at or below LOD (data not shown). Unpaired t-test was used for statistical analysis. *P < 0.05, ****P < 0.0001.