Fig 6.

Comparison of the growth of M. acetivorans CRISPRi repression strains. (A) DJL74 (gRNA-nifH), (B) DJL107 (gRNA-vnfH), (C) DJL120 (gRNA-anfI₁), and (D) DJL119 (gRNA-vnfH/anfI₁) to the control strain DJL72 in the absence of NH₄Cl in HS medium with Mo + Fe (green), V + Fe (blue), or Fe alone (red). In all panels, strain DJL72 is represented by squares and the CRISPRi repression strain by circles. Error bars represent mean ± 1SD from at least three biological replicates. (E) Western blot of lysates from cells of strains DJL72 and DJL74 taken at the timepoints indicated by the arrows in panel (A).