TABLE 2.
Effect of metal and NH4Cl availability on growth of M. acetivorans with methanol
| Relevant metals | Nitrogen source | Lag time (h) a | Generation time (h) b | Cell yield (cells/mL) b |
|---|---|---|---|---|
| Mo + Fe | NH4Cl | 30 | 8.2 ± 0.5 | 3.02 ± 0.2 × 108 |
| N2 | 48 | 28.5 ± 4 | 1.92 ± 0.3 × 108 | |
| V + Fe | NH4Cl | 30 | 8.5 ± 0.1 | 3.08 ± 0.2 × 108 |
| N2 | 90 | 44.5 ± 4.1 | 1.53 ± 0.6 × 108 | |
| Fe-only | NH4Cl | 30 | 8.7 ± 0.1 | 3.34 ± 0.2 × 108 |
| N2 | 96 | 82 ± 4.1 | 9.88 ± 0.4 × 107 |
a
Approximate time when culture entered exponential phase.
b
Generation time and cell yield represent the mean ± 1 SD from at least three biological replicates. Cell yield was determined by cell counts (initial cells/mL = 6.0 x× 106).